Washing and saturating Oil with ultra-pure water avoids critical components of the media, such as hormones and growth factors, getting absorbed into the oil and thus affecting the concentration of the media. Filtering oil ensures that the oil is sterile to ensure highest quality in your IVF system.
Certain stages of bovine in vitro production require an oil overlay to protect against media evaporation, especially when you are incubating for extended periods (i.e. in vitro culture) or working with drops.
Evaporation increases osmolality and affects the overall composition as concentration of media components are increased with loss of evaporated water.
When using an oil overlay, the lid should be placed half open on the 4WP to ensure that equilibration can occur (even 1μl of oil in the lid forms a seal, preventing equilibration with CO2 and all your embryos will die).
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