EQUINE ICSI MEDIA

Our Full Range of Specialised Media

Our ready to use, serum-free EQ-Range provides the complete media system needed to routinely perform equine IVP, from OPU right through to transfer / vitrification.

Conventional equine IVF is not repeatable for efficient embryo production in the way that is observed in other species such as bovine, caprine, ovine and camelid. The reasons for this are not entirely understood but is widely thought to be due to incomplete oocyte maturation and/ or incomplete sperm capacitation.

One way to by-pass these barriers is to inject a single sperm cell directly into the matured oocyte in a procedure known as intracytoplasmic sperm injection (ICSI).

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” The use of IVF Bioscience media has significantly improved our development rates (maturation, cleavage, and blastocyst) and embryo quality for both our equine and bovine in vitro fertilization studies. The use of defined media and the protocols provided by IVF Bioscience have streamlined the in vitro embryo production in our laboratory. 

Dr Leisinger

Chelsey Leisinger, PhD
Adjunct Assistant Professor, LSU School of Veterinary Medicine 


 

Discover our complete equine media suite

The EQ-Range provides a ready to use, serum-free media solution for each step of the equine OPU-IVP protocol.

Our innovative EQ-ICSI kit provides the entire collection of products required for the injection procedure, using biologically degradable components.

EQUINE IVP MEDIA MAP

Our Equine Media Suite Range From Aspiration To Transfer

OPU
Ovum pick-up medium

For performing ovum-pick up and embryo flushing

  • Enriched with vitamins and amino acids to prevent amino acid depletion of oocytes and embryos
  • Does not require refrigeration
  • Supplemented with heparin and gentamycin
Wash
Oocyte and Embryo Wash Medium

For the handling and washing of oocytes and embryos outside of the incubator

  • Enriched with vitamins and amino acids to prevent amino acid depletion of oocytes
  • Supplemented with gentamycin and bicarbonate
  • Does not require CO2 equilibration (HEPES buffered)
EQ-IVM
Oocyte Maturation Medium

For the in vitro maturation of equine oocytes in the laboratory

  • Supplemented with equine-specific gonadotrophic hormones and gentamycin
  • Serum free
  • Suitable for maturation in wells and micro drops
EQ-SemenPrep
Semen Preparation Medium

For the preparation of semen prior to ICSI

  • Non-capacitating formula in preparation for ICSI
  • Dual purpose medium optimised for washing and swim up sperm preparation
  • Avoids the toxicity of traditional gradients; supports a gentler centrifugation process; and allows for fewer steps than traditional processes
  • Contains gentamycin
EQ-ICSI Kit
Equine Intracytoplasmic Sperm Injection Kit

The EQ-ICSI Kit provides the entire collection of media required for the ICSI step, conveniently packaged into a kit of 4 x 1 ml vials:

  • EQ-SpermControl –equine sperm immobilisation medium for selecting a single sperm cell for ICSI. Formulated with a physiological PVP replacement, hyaluronan
  • EQ-Strip – medium to facilitate the removal of cumulus cells from matured oocytes, contains hyaluronidase
  • EQ-PCM – medium for coating the injection pipette to avoid sperm adhering inside the tip
  • EQ-ICSI – HEPES buffered ICSI medium, maintains the pH during the procedure
EQ-IVC-1
Embryo culture two step media

For in vitro culture of equine embryos after ICSI with low glucose

  • Supplemented with BSA, vitamins, amino acids, anti-oxidants and gentamycin
  • Serum free
  • Contains a synthetic serum replacement
  • Suitable for culture in dishes and microdrops with an Oil overlay
EQ-IVC-2
Embryo culture two step media

For in vitro culture of day 5 equine embryos to blastocyst with high glucose

  • Supplemented with BSA, vitamins, amino acids, anti-oxidants and gentamycin
  • Serum free
  • Contains a synthetic serum replacement
  • Suitable for culture in dishes and microdrops with an Oil overlay
EQ-Hold
Oocyte holding medium

For the holding or transportation of oocytes

  • Formulated to hold oocytes at room temperature for up to 24 hours without initiating maturation
  • Does not require CO2 equilibration (HEPES-buffered)
  • Serum free
  • Supplemented with proteins, sodium bicarbonate and gentamycin
Biopsy
Biopsy Medium

For use during embryo biopsy and embryo splitting procedures

  • Provides optimised embryo conditions for handling outside the incubator
  • Supplemented with proteins, sodium bicarbonate and gentamycin
  • Serum free
  • Does not require CO2 equilibration (HEPES buffered)
Transfer
Embryo Transfer and Transportation Medium

For the holding, transportation and transfer of embryos

  • Supports embryo development from day 5 to the blastocyst stage
  • Allows for holding and transportation of developing embryos for up to 24 hours outside the incubator
  • Maintains optimal embryo conditions outside the incubator
  • Suitable for both in vivo and in vitro derived embryos
  • Supplemented with proteins, sodium bicarbonate and gentamycin
  • Serum free
  • Does not require CO2 equilibration (HEPES buffered)

Transfer can also be used to incubate In Vivo Embryos; allowing users to confirm embryo viability, optimise recipients and gain extra pregnancies from lower grade embryos*

*Demetrio, D. (2016). THE USE OF IN VITRO CULTURE MEDIA TO INCUBATE LOW-QUALITY IN-VIVO PRODUCED EMBRYOS. In: 2016 AETA & CETA/ACTE Joint Convention Proceedings. St Louis, MO, pp 51-52.
*Engelland, G. (2017). PRACTICE TIP: INCUBATING IN VIVO EMBRYOS. In: 2017 AETA & CETA/ACTE Joint Convention Proceedings. Orlando, FL, pp 34.

EQ-VitriCool
Vitrification Cooling Media Kit

For the vitrification of equine embryos and oocytes

  • Suitable for both in vivo and in vitro derived equine embryos
  • Ethylene glycol and DMSO based formula
  • Serum free
  • Designed to be use in conjunction with the EQ-VitriWarm kit for warming and recovery of embryos and oocytes

EQ-VitriCool is a 3-step cooling media kit comprised of 1 x 5 ml bottle, and 2 x 1 ml bottles.

EQ-VitriWarm
Vitrification Warming Media Kit

For the warming of equine embryos and oocytes following vitrification

  • Suitable for both in vivo and in vitro derived equine embryos
  • Supplemented with macromolecules such as sucrose and albumin to help reduce physical damage and help maintain osmotic pressure of the extracellular fluid
  • Serum free
  • Designed to be used in conjunction with the EQ-VitriCool kit for vitrification of embryos and oocytes

EQ-VitriWarm is a 4-step warming media kit comprised of 1 x 5 ml bottle, and 3 x 1 ml bottles.

Oil
Oil for Medium Overlay

Oil to overlay media

  • Highly refined, pharmaceutical grade liquid paraffin (light mineral) oil
  • Pre-washed twice with ultra-pure water
  • Sterilised via filtration through a 0.2 µm filter
  • Each batch undergoes strict quality control assessments for sterility, density, viscosity, endotoxins, and peroxide value (POV)
  • Each batch is mouse embryo assay (MEA) and bovine embryo assay (BEA) tested

See All Publications

Read Posters, Papers and Abstracts referencing IVF Bioscience media.

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Certificates

Lot-specific Certificates of Analysis

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Ref No Name Description Size
81001 EQ-IVM Oocyte Maturation Medium 10ml
81002 EQ-SemenPrep Semen preparation medium 20ml
81003 EQ-ICSI Kit Equine Intracytoplasmic Sperm Injection Kit  4 x 1ml
81004 EQ-IVC-1 Embryo culture two step media 10ml
81005 EQ-IVC-2 Embryo culture two step media 10ml
81006 EQ-Hold Oocyte holding medium 10ml
81007 EQ-VitriCool Kit Vitrification Cooling Media Kit Kit
81008 EQ-VitriWarm Kit Vitrification Warming Media Kit Kit
51001 OPU Ovum pick-up medium 500ml
51002 Wash Oocyte and embryo wash medium 50ml
51003 Oil Oil for medium overlay 50ml
51004 Transfer Embryo transfer and transportation medium 10ml
51005 Biopsy Biopsy medium 10ml