BOVINE MEDIA

A complete, optimised media system

From OPU to transfer, we provide ready to use*, serum-free media for every step in the in vitro production (IVP) process.

Each product is optimised to provide enhanced results and convenience, which is further boosted by their synergy when used together.

Arrange Your Trial

” We have found IVF Bioscience media to be very effective in supporting in vitro bovine embryo development to blastocyst. We observe excellent cumulus expansion during IVM and high cleavage rates (over 75% cleaved) following IVF.

Blastocyst production using the BO system from IVF Bioscience is high (over 50% blastocysts from cleaved), and the embryos are of good quality with many embryos hatching in vitro. We have been extremely pleased with how this system has worked in our laboratory. “

Rebecca Headshot

Rebecca Krisher, PhD
Research Director at CCRM Research


 

*Due to import regulations, some products shipped to Australia and New Zealand will be BSA-free, where BSA supplementation will be required by the end user.

Our BO-Range is suitable for bovine, camelid, ovine and caprine species

Discover our complete bovine media suite

Media Synergy Results

Proven Synergy Between Media

Superior results are achieved by using the entire media system due to their synergy when used together

This study investigated the performance of IVF Bioscience media as a system compared to their use as stand-alone products with TCM199/SOF.

The use of BO-IVM, BO-IVF, BO-IVC and BO-SemenPrep together as a system produced improved blastocyst rates and higher kinetic and morphology scores than using the products in isolation.

Read the publication

“Over 45% blastocyst rates in sheep and high pregnancy rates too”

“Great cleavage and blast rates”

“Many caprine embryo transfers from IVF with positive ultrasounds”

“I have been testing IVF Bioscience media and one other source. I had very good success with my first round with goat ovaries using IVF Bioscience media and haven’t had as much success with the competitor. I really do prefer IVF Bioscience media.”

Dawn Bresnahan

Dawn Bresnahan, MS, PhD. Assistant Professor
Dept. of Animal Science at Berry College


 

Improved Results

The IVF Bioscience media system produced in vitro embryos with higher mean cell counts and greater tolerance to seasonal temperature fluctuations.

This study compared IVF Bioscience media (ETB – BO-IVM, BO-IVF and BO-IVC) with a control system. In comparison to the control, IVF Bioscience media was shown to produce a greater number of viable embryos, higher mean cell counts, and far greater consistency in embryo viability.

Read the publication

” We have used IVF Bioscience media for production of IVF embryos and we have been getting excellent results. Twenty-eight cows have so far been impregnated, with embryos developed with ova from four high-yielding indigenous breeds.”

Dr Shyam Zawar
CEO at JK Bovagenix


 

Read more about Dr Zawar’s experience of IVF Bioscience media in our news story: India’s first frozen IVF calves

Read More

BOVINE IVP MEDIA MAP

Our Bovine Media Suite Range From Aspiration To Transfer

OPU
Ovum pick-up medium

For performing ovum-pick up and embryo flushing

  • Enriched with vitamins and amino acids to prevent amino acid depletion of oocytes and embryos
  • Does not require refrigeration
  • Supplemented with heparin and gentamycin
Wash
Oocyte and Embryo Wash Medium

For the handling and washing of oocytes and embryos outside of the incubator

  • Enriched with vitamins and amino acids to prevent amino acid depletion of oocytes
  • Supplemented with gentamycin and bicarbonate
  • Does not require CO2 equilibration (HEPES buffered)
BO-IVM
Oocyte Maturation Medium

For the in vitro maturation of oocytes in the laboratory

  • Serum free
  • Suitable for maturation in dishes and micro drops
  • Supplemented with low glucose, gonadotrophic hormones and gentamycin

HEPES-buffered form also available for maturation away from the laboratory (BO-HEPES-IVM).

BO-HEPES-IVM
HEPES-Buffered Oocyte Maturation Medium

For the in vitro maturation of oocytes outside the incubator

  • Allows for maturation of oocytes away from the laboratory and during transportation without a CO2 environment
  • Also suitable for traditional use in incubators
  • Supplemented with low glucose, gonadotrophic hormones and gentamycin
  • Serum free
  • Does not require CO2 equilibration (HEPES buffered)
  • Supplied with our pH indicator guide for easy identification of pH by colour

Due to import regulations, BO-HEPES-IVM shipped to Australia and New Zealand will be BSA-free, where BSA supplementation will be required by the end user.

BO-SemenPrep
Semen Preparation Medium

For the washing of semen prior to in vitro fertilisation

  • Non-capacitating formula
  • Conserves spermatozoa energy prior to introduction to the fertilisation medium
  • Avoids the toxicity of traditional gradients
  • Supports a gentler centrifugation process
  • Allows for fewer steps than traditional procedures, minimising potential cell damage
  • Contains gentamycin
BO-IVF
Fertilisation Medium

For the in vitro fertilisation of matured oocytes

  • Serum free
  • Supplemented with heparin and gentamycin
  • Enriched with an optimised PHE solution for sperm capacitation, improved pronuclear formation and enhanced embryonic development

Due to import regulations, BO-IVF shipped to Australia and New Zealand will be BSA-free, where BSA supplementation will be required by the end user.

BO-IVC
Embryo Culture Medium

For the in vitro culture of inseminated oocytes

  • Non-sequential, single step system which does not require a change of medium
  • Suitable for continuous culture from zygote to blastocyst stage
  • Serum free
  • Contains a synthetic serum replacement
  • Supplemented with BSA, vitamins, amino acids, anti-oxidants and gentamycin
  • Enriched with hyaluronate for increased cryo-survivability
  • Suitable for culture in dishes and micro drops with an Oil overlay

Due to import regulations, BO-IVC shipped to Australia and New Zealand will be BSA-free, where BSA supplementation will be required by the end user.

Biopsy
Biopsy Medium

For use during embryo biopsy and embryo splitting procedures

  • Provides optimised embryo conditions for handling outside the incubator
  • Supplemented with proteins, sodium bicarbonate and gentamycin
  • Serum free
  • Does not require CO2 equilibration (HEPES buffered)

Due to import regulations, Biopsy shipped to Australia and New Zealand will be BSA-free, where BSA supplementation will be required by the end user.

Transfer
Embryo Transfer and Transportation Medium

For the holding, transportation and transfer of embryos

  • Supports embryo development from day 5 to the blastocyst stage
  • Allows for holding and transportation of developing embryos for up to 24 hours outside the incubator
  • Maintains optimal embryo conditions outside the incubator
  • Suitable for both in vivo and in vitro derived embryos
  • Supplemented with proteins, sodium bicarbonate and gentamycin
  • Serum free
  • Does not require CO2 equilibration (HEPES buffered)

Transfer can also be used to incubate In Vivo Embryos; allowing users to confirm embryo viability, optimise recipients and gain extra pregnancies from lower grade embryos*

*Demetrio, D. (2016). THE USE OF IN VITRO CULTURE MEDIA TO INCUBATE LOW-QUALITY IN-VIVO PRODUCED EMBRYOS. In: 2016 AETA & CETA/ACTE Joint Convention Proceedings. St Louis, MO, pp 51-52.
*Engelland, G. (2017). PRACTICE TIP: INCUBATING IN VIVO EMBRYOS. In: 2017 AETA & CETA/ACTE Joint Convention Proceedings. Orlando, FL, pp 34.

Due to import regulations, Transfer shipped to Australia and New Zealand will be BSA-free, where BSA supplementation will be required by the end user.

BO-VitriCool Kit
Vitrification Cooling 3-step Media Kit

Vitricool-group

For the vitrification of embryos

  • Suitable for both in vivo and in vitro derived embryos
  • Ethylene glycol and DMSO based formula
  • Serum free
  • Designed to be used in conjunction with the BO-VitriWarm kit for warming and recovery of embryos

BO-VitriCool is a 3-step cooling media kit comprised of 1 x 5 ml bottle, and 2 x 1 ml bottles.

BO-VitriWarm Kit
Vitrification Warming 4-step Media Kit

Vitriwarm-group

For the warming of embryos following vitrification

  • Suitable for both in vivo and in vitro derived embryos
  • Supplemented with macromolecules such as sucrose and albumin to help reduce physical damage and help maintain osmotic pressure of the extracellular fluid
  • Serum free
  • Designed to be used in conjunction with the BO-VitriCool kit for vitrification of embryos

BO-VitriWarm is a 4-step warming media kit comprised of 1 x 5 ml bottle, and 3 x 1 ml bottles.

Oil
Oil for Medium Overlay

Oil to overlay media

  • Highly refined, pharmaceutical grade liquid paraffin (light mineral) oil
  • Pre-washed twice with ultra-pure water
  • Sterilised via filtration through a 0.2µm filter
  • Each batch undergoes strict quality control assessments for sterility, density, viscosity, endotoxins, and peroxide value (POV)
  • Each batch is mouse embryo assay (MEA) and bovine embryo assay (BEA) tested

See All Publications

Read Posters, Papers and Abstracts referencing IVF Bioscience media.

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Certificates

Lot-specific Certificates of Analysis

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Request a copy of our protocol pack by completing the form below:

Protocols of Interest, tick all that apply *
Bovine
Bovine On-site commercial protocolBovine Off-site commercial protocolBovine Slaughterhouse trial protocolBovine Vitrification protocol
Equine
Equine Slaughterhouse trial protocolEquine Commercial protocolEquine Vitrification protocol

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Ref No Name Description Size
71001 BO-IVM Oocyte maturation medium 10ml
71002 BO-HEPES-IVM HEPES-buffered oocyte maturation medium 10ml
71003 BO-SemenPrep Semen preparation medium 20ml
71004 BO-IVF Fertilisation medium 10ml
71005 BO-IVC Embryo culture medium 10ml
71006 BO-VitriCool Vitrification cooling media kit Kit
71007 BO-VitriWarm Vitrification warming media kit Kit
51001 OPU Ovum pick-up medium 500ml
51002 Wash Oocyte and embryo wash medium 50ml
51003 Oil Oil for medium overlay 50ml
51004 Transfer Embryo transfer and transportation medium 10ml
51005 Biopsy Biopsy medium 10ml