BOVINE MEDIA
A complete, optimised media system
From OPU to transfer, we provide ready to use*, serum-free media for every step in the in vitro production (IVP) process.
Each product is optimised to provide enhanced results and convenience, which is further boosted by their synergy when used together.
” We have found IVF Bioscience media to be very effective in supporting in vitro bovine embryo development to blastocyst. We observe excellent cumulus expansion during IVM and high cleavage rates (over 75% cleaved) following IVF.
Blastocyst production using the BO system from IVF Bioscience is high (over 50% blastocysts from cleaved), and the embryos are of good quality with many embryos hatching in vitro. We have been extremely pleased with how this system has worked in our laboratory. “
Rebecca Krisher, PhD
Research Director at CCRM Research
*Due to import regulations, some products shipped to Australia and New Zealand will be BSA-free, where BSA supplementation will be required by the end user.
Our BO-Range is suitable for bovine, camelid, ovine and caprine species
Discover our complete bovine media suite
Proven Synergy Between Media
Superior results are achieved by using the entire media system due to their synergy when used together
This study investigated the performance of IVF Bioscience media as a system compared to their use as stand-alone products with TCM199/SOF.
The use of BO-IVM, BO-IVF, BO-IVC and BO-SemenPrep together as a system produced improved blastocyst rates and higher kinetic and morphology scores than using the products in isolation.
“Over 45% blastocyst rates in sheep and high pregnancy rates too”
“Great cleavage and blast rates”
“Many caprine embryo transfers from IVF with positive ultrasounds”
“I have been testing IVF Bioscience media and one other source. I had very good success with my first round with goat ovaries using IVF Bioscience media and haven’t had as much success with the competitor. I really do prefer IVF Bioscience media.”
Dawn Bresnahan, MS, PhD. Assistant Professor
Dept. of Animal Science at Berry College
Improved Results
The IVF Bioscience media system produced in vitro embryos with higher mean cell counts and greater tolerance to seasonal temperature fluctuations.
This study compared IVF Bioscience media (ETB – BO-IVM, BO-IVF and BO-IVC) with a control system. In comparison to the control, IVF Bioscience media was shown to produce a greater number of viable embryos, higher mean cell counts, and far greater consistency in embryo viability.
” We have used IVF Bioscience media for production of IVF embryos and we have been getting excellent results. Twenty-eight cows have so far been impregnated, with embryos developed with ova from four high-yielding indigenous breeds.”
Dr Shyam Zawar
CEO at JK Bovagenix
Read more about Dr Zawar’s experience of IVF Bioscience media in our news story: India’s first frozen IVF calves
BOVINE IVP MEDIA MAP
Our Bovine Media Range From Aspiration To Transfer
Ovum pick-up medium
For performing ovum-pick up and embryo flushing
- Enriched with vitamins and amino acids to prevent amino acid depletion of oocytes and embryos
- Does not require refrigeration
- Supplemented with heparin and gentamycin
Oocyte and Embryo Wash Medium
For the handling and washing of oocytes and embryos outside of the incubator
- Enriched with vitamins and amino acids to prevent amino acid depletion of oocytes
- Supplemented with gentamycin and bicarbonate
- Does not require CO2 equilibration (HEPES buffered)
Oocyte Maturation Medium
For the in vitro maturation of oocytes in the laboratory
- Serum free
- Suitable for maturation in dishes and micro drops
- Supplemented with low glucose, gonadotrophic hormones and gentamycin
HEPES-buffered form also available for maturation away from the laboratory (BO-HEPES-IVM).
HEPES-Buffered Oocyte Maturation Medium
For the in vitro maturation of oocytes outside the incubator
- Allows for maturation of oocytes away from the laboratory and during transportation without a CO2 environment
- Also suitable for traditional use in incubators
- Supplemented with low glucose, gonadotrophic hormones and gentamycin
- Serum free
- Does not require CO2 equilibration (HEPES buffered)
- Supplied with our pH indicator guide for easy identification of pH by colour
Due to import regulations, BO-HEPES-IVM shipped to Australia and New Zealand will be BSA-free, where BSA supplementation will be required by the end user.
Semen Preparation Medium
For the washing of semen prior to in vitro fertilisation
- Non-capacitating formula
- Conserves spermatozoa energy prior to introduction to the fertilisation medium
- Avoids the toxicity of traditional gradients
- Supports a gentler centrifugation process
- Allows for fewer steps than traditional procedures, minimising potential cell damage
- Contains gentamycin
Fertilisation Medium
For the in vitro fertilisation of matured oocytes
- Serum free
- Supplemented with heparin and gentamycin
- Enriched with an optimised PHE solution for sperm capacitation, improved pronuclear formation and enhanced embryonic development
Due to import regulations, BO-IVF shipped to Australia and New Zealand will be BSA-free, where BSA supplementation will be required by the end user.
Embryo Culture Medium
For the in vitro culture of inseminated oocytes
- Non-sequential, single step system which does not require a change of medium
- Suitable for continuous culture from zygote to blastocyst stage
- Serum free
- Contains a synthetic serum replacement
- Supplemented with BSA, vitamins, amino acids, anti-oxidants and gentamycin
- Enriched with hyaluronate for increased cryo-survivability
- Suitable for culture in dishes and micro drops with an Oil overlay
Due to import regulations, BO-IVC shipped to Australia and New Zealand will be BSA-free, where BSA supplementation will be required by the end user.
Biopsy Medium
For use during embryo biopsy and embryo splitting procedures
- Provides optimised embryo conditions for handling outside the incubator
- Supplemented with proteins, sodium bicarbonate and gentamycin
- Serum free
- Does not require CO2 equilibration (HEPES buffered)
Due to import regulations, Biopsy shipped to Australia and New Zealand will be BSA-free, where BSA supplementation will be required by the end user.
Embryo Transfer and Transportation Medium
For the holding, transportation and transfer of embryos
- Supports embryo development from day 5 to the blastocyst stage
- Allows for holding and transportation of developing embryos for up to 24 hours outside the incubator
- Maintains optimal embryo conditions outside the incubator
- Suitable for both in vivo and in vitro derived embryos
- Supplemented with proteins, sodium bicarbonate and gentamycin
- Serum free
- Does not require CO2 equilibration (HEPES buffered)
Transfer can also be used to incubate In Vivo Embryos; allowing users to confirm embryo viability, optimise recipients and gain extra pregnancies from lower grade embryos*
*Demetrio, D. (2016). THE USE OF IN VITRO CULTURE MEDIA TO INCUBATE LOW-QUALITY IN-VIVO PRODUCED EMBRYOS. In: 2016 AETA & CETA/ACTE Joint Convention Proceedings. St Louis, MO, pp 51-52.
*Engelland, G. (2017). PRACTICE TIP: INCUBATING IN VIVO EMBRYOS. In: 2017 AETA & CETA/ACTE Joint Convention Proceedings. Orlando, FL, pp 34.
Due to import regulations, Transfer shipped to Australia and New Zealand will be BSA-free, where BSA supplementation will be required by the end user.
Vitrification Cooling 3-step Media Kit
For the vitrification of embryos
- Suitable for both in vivo and in vitro derived embryos
- Ethylene glycol and DMSO based formula
- Serum free
- Designed to be used in conjunction with the BO-VitriWarm kit for warming and recovery of embryos
BO-VitriCool is a 3-step cooling media kit comprised of 1 x 5 ml bottle, and 2 x 1 ml bottles.
Vitrification Warming 4-step Media Kit
For the warming of embryos following vitrification
- Suitable for both in vivo and in vitro derived embryos
- Supplemented with macromolecules such as sucrose and albumin to help reduce physical damage and help maintain osmotic pressure of the extracellular fluid
- Serum free
- Designed to be used in conjunction with the BO-VitriCool kit for vitrification of embryos
BO-VitriWarm is a 4-step warming media kit comprised of 1 x 5 ml bottle, and 3 x 1 ml bottles.
Oil for Medium Overlay
Oil to overlay media
- Highly refined, pharmaceutical grade liquid paraffin (light mineral) oil
- Pre-washed twice with ultra-pure water
- Sterilised via filtration through a 0.2µm filter
- Each batch undergoes strict quality control assessments for sterility, density, viscosity, endotoxins, and peroxide value (POV)
- Each batch is mouse embryo assay (MEA) tested at an independent testing laboratory
Ref No | Name | Description | Size |
---|---|---|---|
71001 | BO-IVM™ | Oocyte maturation medium | 10ml |
71002 | BO-HEPES-IVM™ | HEPES-buffered oocyte maturation medium | 10ml |
71003 | BO-SemenPrep™ | Semen preparation medium | 20ml |
71004 | BO-IVF™ | Fertilisation medium | 10ml |
71005 | BO-IVC™ | Embryo culture medium | 10ml |
71006 | BO-VitriCool™ | Vitrification cooling media kit | Kit |
71007 | BO-VitriWarm™ | Vitrification warming media kit | Kit |
51001 | OPU | Ovum pick-up medium | 500ml |
51002 | Wash | Oocyte and embryo wash medium | 50ml |
51003 | Oil | Oil for medium overlay | 50ml |
51004 | Transfer | Embryo transfer and transportation medium | 10ml |
51005 | Biopsy | Biopsy medium | 10ml |