BOVINE MEDIA

For performing ovum-pick up and embryo flushing

• Enriched with vitamins and amino acids to prevent amino acid depletion of oocytes and embryos
• Does not require refrigeration
• Supplemented with heparin and gentamycin

For the handling and washing of oocytes and embryos outside of the incubator

• Enriched with vitamins and amino acids to prevent amino acid depletion of oocytes
• Supplemented with gentamycin and bicarbonate
• Does not require CO₂ equilibration (HEPES buffered)

For the in vitro maturation of oocytes in the laboratory

• Serum free
• Suitable for maturation in dishes and micro drops
• Supplemented with low glucose, gonadotrophic hormones and gentamycin

HEPES-buffered form also available for maturation away from the laboratory (BO-HEPES-IVM).

For the in vitro maturation of oocytes outside the incubator

• Allows for maturation of oocytes away from the laboratory and during transportation without a CO₂ environment
• Also suitable for traditional use in incubators
• Supplemented with low glucose, gonadotrophic hormones and gentamycin
• Serum free
• Does not require CO₂ equilibration (HEPES buffered)
• Supplied with our pH indicator guide for easy identification of pH by colour

Non HEPES-buffered maturation medium is also available (BO-IVM).

For the washing of semen prior to in vitro fertilisation

• Non-capacitating formula
• Conserves spermatozoa energy prior to introduction to the fertilisation medium
• Avoids the toxicity of traditional gradients
• Supports a gentler centrifugation process
• Allows for fewer steps than traditional procedures, minimising potential cell damage
• Contains gentamycin

For the in vitro fertilisation of matured oocytes

• Serum free
• Supplemented with heparin and gentamycin
• Enriched with an optimised PHE solution for sperm capacitation, improved pronuclear formation and enhanced embryonic development

For the in vitro culture of inseminated oocytes

• Non-sequential, single step system which does not require a change of medium
• Suitable for continuous culture from zygote to blastocyst stage
• Serum free
• Contains a synthetic serum replacement
• Supplemented with BSA, vitamins, amino acids, anti-oxidants and gentamycin
• Enriched with hyaluronate for increased cryo-survivability
• Suitable for culture in dishes and micro drops with an Oil overlay

For use during embryo biopsy and embryo splitting procedures

• Provides optimised embryo conditions for handling outside the incubator
• Supplemented with proteins, sodium bicarbonate and gentamycin
• Serum free
• Does not require CO₂ equilibration (HEPES buffered)

For the holding, transportation and transfer of embryos

• Supports embryo development from day 5 to the blastocyst stage
• Allows for holding and transportation of developing embryos for up to 24 hours outside the incubator
• Maintains optimal embryo conditions outside the incubator
• Suitable for both in vivo and in vitro derived embryos
• Supplemented with proteins, sodium bicarbonate and gentamycin
• Serum free
• Does not require CO₂ equilibration (HEPES buffered)

Transfer can also be used to incubate In Vivo Embryos; allowing users to confirm embryo viability, optimise recipients and gain extra pregnancies from lower grade embryos*

*Demetrio, D. (2016). THE USE OF IN VITRO CULTURE MEDIA TO INCUBATE LOW-QUALITY IN-VIVO PRODUCED EMBRYOS. In: 2016 AETA & CETA/ACTE Joint Convention Proceedings. St Louis, MO, pp 51-52.
*Engelland, G. (2017). PRACTICE TIP: INCUBATING IN VIVO EMBRYOS. In: 2017 AETA & CETA/ACTE Joint Convention Proceedings. Orlando, FL, pp 34.

For the vitrification of embryos

• Suitable for both in vivo and in vitro derived embryos
• Ethylene glycol and DMSO based formula
• Serum free
• Designed to be used in conjunction with the BO-VitriWarm kit for warming and recovery of embryos

BO-VitriCool is a 3-step cooling media kit comprised of 1 x 5 ml bottle, and 2 x 1 ml bottles.

For the warming of embryos following vitrification

• Suitable for both in vivo and in vitro derived embryos
• Supplemented with macromolecules such as sucrose and albumin to help reduce physical damage and help maintain osmotic pressure of the extracellular fluid
• Serum free
• Designed to be used in conjunction with the BO-VitriCool kit for vitrification of embryos

BO-VitriWarm is a 4-step warming media kit comprised of 1 x 5 ml bottle, and 3 x 1 ml bottles.

Oil to overlay media

• Highly refined, pharmaceutical grade liquid paraffin (light mineral) oil
• Pre-washed twice with ultra-pure water
• Sterilised via filtration through a 0.2µm filter
• Each batch undergoes strict quality control assessments for sterility, density, viscosity, endotoxins, and peroxide value (POV)
• Each batch is mouse embryo assay (MEA) and bovine embryo assay (BEA) tested