In collaboration with the Advanced Breeding Team at Tyndale Veterinary Practice Ltd. U.K., IVF Bioscience welcomed delegates from the EU and the UK, to be trained in the skills to successfully develop in vitro produced (IVP) bovine embryos; from immature oocytes right up to the blastocyst stage.
Attendees were invited to experience first-hand the day-to-day running of a successful commercial bovine OPU-IVP provider and received hands-on training covering each aspect of the IVP protocol, as well as some theoretical classroom sessions.
Bovine IVF Course Structure
Firstly, attendees were given a tour of the facilities and introduced to Quality Assurance and Laboratory Management. Slaughterhouse ovaries were then aspirated before the oocytes were searched in a dish and cultured in maturation medium overnight.
Following overnight culture, oocyte maturation was assessed via cumulus cell expansion and viscoelasticity.
Sperm quality (from both conventional and sex-sorted straws) was also assessed for motility and concentration, before being washed and introduced to the matured oocytes, for overnight fertilisation.
Meanwhile, blastocysts that had been cultured one week prior to the course were assessed and graded in accordance with the IETS guidelines.
Attendees were then introduced to the importance of record keeping and labelling for commercial IVF embryos, to comply with UK regulations. The blastocysts from the previous week were loaded into straws, so that they could be slow-frozen prior to being stored in liquid nitrogen.
Presumptive zygotes from the previous day were assessed for fertilisation rates and denuded of their cumulus cells prior to being cultured in our non-sequential culture medium, BO-IVC, for seven days.
The purpose of the bovine IVF training course was to introduce the basic aspects of IVP, upskill attendees’ laboratory skills and improve their confidence in handling gametes and embryos in the IVF laboratory.
The course was well received, and attendees felt that the sessions equipped them with the knowledge and skills to further their understanding of OPU-IVP and support the use of these technologies in their respective countries.
As the demand for bovine IVP training increases, IVF Bioscience is looking forward to hosting training courses in collaboration with Tyndale Veterinary Practice in the upcoming months. There are still limited spaces available on our 2022 training courses, so book your place today.
What did attendees think of the course?
All delegates rated the course as “Excellent” in terms of organisation, content, and overall satisfaction. As one of the delegates commented, “The whole IVF laboratory course offered was extremely useful for us”.
Privacy & Cookies Policy
Necessary cookies are absolutely essential for the website to function properly. This category only includes cookies that ensures basic functionalities and security features of the website. These cookies do not store any personal information.
Any cookies that may not be particularly necessary for the website to function and is used specifically to collect user personal data via analytics, ads, other embedded contents are termed as non-necessary cookies. It is mandatory to procure user consent prior to running these cookies on your website.
Functional cookies help to perform certain functionalities like sharing the content of the website on social media platforms, collect feedbacks, and other third-party features.
This cookie is set by HubSpot. The purpose of the cookie is to keep track of sessions. This is used to determine if HubSpot should increment the session number and timestamps in the __hstc cookie. It contains the domain, viewCount (increments each pageView in a session), and session start timestamp.
1 year 24 days
This cookie is set by hubspot. This cookie is used to recognize the user who have chatted using the messages tool. This cookies is stored if the user leaves before they are added as a contact. If the returning user visits again with this cookie on the browser, the chat history with the user will be loaded.
Analytical cookies are used to understand how visitors interact with the website. These cookies help provide information on metrics the number of visitors, bounce rate, traffic source, etc.
1 year 24 days
This cookie is set by Hubspot and is used for tracking visitors. It contains the domain, utk, initial timestamp (first visit), last timestamp (last visit), current timestamp (this visit), and session number (increments for each subsequent session).
This cookie is installed by Google Analytics. The cookie is used to calculate visitor, session, campaign data and keep track of site usage for the site's analytics report. The cookies store information anonymously and assign a randomly generated number to identify unique visitors.
This cookie is installed by Google Analytics. The cookie is used to store information of how visitors use a website and helps in creating an analytics report of how the website is doing. The data collected including the number visitors, the source where they have come from, and the pages visted in an anonymous form.
1 year 24 days
This cookie is used by HubSpot to keep track of the visitors to the website. This cookie is passed to Hubspot on form submission and used when deduplicating contacts.
Advertisement cookies are used to provide visitors with relevant ads and marketing campaigns. These cookies track visitors across websites and collect information to provide customized ads.
1 year 24 days
Used by Google DoubleClick and stores information about how the user uses the website and any other advertisement before visiting the website. This is used to present users with ads that are relevant to them according to the user profile.
This cookie is set by doubleclick.net. The purpose of the cookie is to determine if the user's browser supports cookies.
5 months 27 days
This cookie is set by Youtube. Used to track the information of the embedded YouTube videos on a website.