Autumn sunshine at the AETA-CETA/ACTE meeting

AETA 2017 Hotel

At the end of October 2017 the IVF Bioscience team joined the 300+ members of the AETA and ACTE/CETA societies heading to Orlando for their joint annual convention. The meeting saw an action-packed two-day itinerary, with great talks, top company, and some ‘magical’ evening entertainment!

Workshop: Q & A and Resources

The conference provided a great platform for the presentation our Bovine IVF Workshop, which concentrated on revealing what really goes on inside the “Black Box of IVF” – the steps between OPU and transfer. The session featured custom-made demonstrational videos for each step in the process, which were presented alongside the expert advice of speakers Jane Pryor and Dr. Lotte Stroebech. We received some great feedback, with comments such as “a great overview of the entire process”, “informative IVF lab setup recommendations” and “a lot of solutions to everyday problems”.

Pryor & Stroebech Speakers

The speakers received plenty of opportunities for questions from those in attendance, with popular topics including:

Should IVF embryos be treated differently to MOET embryos?

IVF embryos are more sensitive to their environment than those derived in vivo. It is therefore important to minimise fluctuations in temperature, pH and osmolality, as these are particularly harmful to IVF embryos.

How do you measure and maintain the pH of the media?

The temperature and pH of the medium are critical to IVF success, however you do not have to be able to directly measure the pH to be confident of the pH level.

The pH of the medium is determined by the relationship between bicarbonate concentration, atmospheric CO2 concentration, and altitude. As long as altitude and exact gas concentration within the incubator are both known, the pH can also be calculated.

For IVF Bioscience media, this means that working with 6% CO2 will give the optimal medium pH at sea level. And at 1600m above sea level this would need to be upped to 7.1% CO2 to account for the lower atmospheric pressure. The same modifications would need to be made for the O2 concentration in the culture step. Anything below 5% is detrimental to embryo culture, so it is critical that these concentrations are accurately known and the altitude adjustments are made.

So, to be certain of your pH, measure the gas concentrations and temperature within the incubator, rather than trust the display.

A full chart of altitude adjustments is included in our protocols – get in touch to request further details.

Is it really possible to run my own IVF lab?

In a word, yes! With the right training, equipment and attitude, great results can be achieved with even the most basic of laboratory facilities.

A big part of what IVF Bioscience can offer your business is the support and guidance to make running your own IVF lab a reality. Get in touch today to start your journey towards independent IVF embryo production.

As ever with such workshops, there are always more questions to be asked, so if you have one of your own, feel free to contact the team, and we’ll get you the answers you need.

The resources from the workshop are also now available to access electronically. Just drop us an email to request a copy of the handout, and click through to request access to the protocol videos.

Practice Tip: Incubating In Vivo Embryos

Another highlight of the meeting was the talk presented by Dr. Glenn Engelland concerning the use of BO-Transfer medium for the incubation of MOET-derived embryos. His results have been encouraging, with two practical applications for the technique:

Incubating grade 3 embryos in a straw of BO-Transfer for 24 hours allows their viability to be reliably determined, which in turn leads to transferring only those that have successfully developed.

Incubating all embryos from a flush in straws of BO-Transfer increases work-flow flexibility by allowing the fresh transfers to be conducted the day after flushing.

Full details of Dr. Engelland’s findings can be found in the Joint Convention Proceedings from the meeting on page 34.

We’re here to help – If you’ve been inspired to try IVF and would like to learn more about our consulting and training options; are already conducting IVF and are considering switching to our media; or would like to try some BO-Transfer for incubating ET embryos, then the next step is get in touch – just fill out our contact form and one of the team will respond to you soon.

IVF Bioscience would like to thank Jane Pryor and Dr. Lotte Stroebech for their wonderful work in the workshop, Dr. Glenn Engelland for sharing his findings, and the organising committee – particularly Dr. Charles Looney – for putting together this year’s conference. We look forward to seeing you all again at the 2018 Joint Annual Convention in Montreal!

AETA Stand Team